Department of Clinical Laboratory, Kyoto University Hospital

[6353 / 4D018]

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Ver.17

4.Endocrinological Test >> 4D.Adrenocortical Hormones and Their Binding Protein>>4D018 17-ketosteroids, 7 fractionation [pooled urine]

17-ketosteroids, 7 fractionation

[pooled urine]

0

Standard Code (JLAC10)

1	Analyte	4D018	17-ketosteroids, 7 fractionation
	Identification	0000
	Specimen	004	pooled urine
	Method	203	gas chromatography-mass spectrometry (GC-MS)
Result Identifying 01		4D018	51	androsterone
Result Identifying 02		4D018	52	ethiocholanolone
Result Identifying 03		4D018	53	dehydroepiandrosterone
Result Identifying 04		4D018	54	11-ketoandrosterone
Result Identifying 05		4D018	55	11-ketoethiocholanolone
Result Identifying 06		4D018	56	11-hydroxyandrosterone
Result Identifying 07		4D018	57	11-hydroxyethiocholanolone

Specimen

Top	Colour	Collected Sample	Quantity	Measured Material	Required
White Top		urine per day (24h)	9 mL		5.0 mL

Specimen Rejection Criteria

haemolysis
clotted
lipaemia
inappropriate sample volume
wrong container
urine samples without the appropriate preservative	reject
received ambient
unprotected from light
opening tubes
incterus

Specimen Stability Information

Specimen Type	Container	Temperature	Stability
pooled urine		Refrigerated	4 weeks

Turnaround Time (TAT)

5 - 7 days

Laboratory

outsourcing (LSI Mediennce Corporation)weekday (8:30 a.m. - 5:00 p.m.)
night time or weekend

Reference Value

Period / Age

		Male	Female	Unit
Specimen		pooled urine
Method		gas chromatography-tandem mass spectrometry (GC-MS/MS)
51	androsterone	1.12 - 4.71	0.22 - 2.78	mg/d
52	ethiocholanolone	0.43 - 3.23	0.10 - 2.39	mg/d
53	dehydroepiandrosterone	≤ 2.92	≤ 1.49	mg/d
54	11-ketoandrosterone	≤ 0.50	≤ 0.48	mg/d
55	11-ketoethiocholanolone	0.08 - 0.63	≤ 0.62	mg/d
56	11-hydroxyandrosterone	0.39 - 2.04	0.19 - 1.17	mg/d
57	11-hydroxyethiocholanolone	≤ 0.54	≤ 0.75	mg/d

		Male	Female	Unit
Specimen		pooled urine
Method		gas chromatography-mass spectrometry (GC-MS)
51	androsterone	1.12 - 4.71	0.22 - 2.78	mg/d
52	ethiocholanolone	0.43 - 3.23	0.10 - 2.39	mg/d
53	dehydroepiandrosterone	≤ 2.92	≤ 1.49	mg/d
54	11-ketoandrosterone	≤ 0.50	≤ 0.48	mg/d
55	11-ketoethiocholanolone	0.08 - 0.63	≤ 0.62	mg/d
56	11-hydroxyandrosterone	0.39 - 2.04	0.19 - 1.17	mg/d
57	11-hydroxyethiocholanolone	≤ 0.54	≤ 0.75	mg/d

		Male	Female	Unit
Specimen		pooled urine
Method		gas chromatography-mass spectrometry (GC-MS)
51	androsterone	1.10 - 4.20	0.40 - 3.00	mg/d
52	ethiocholanolone	0.55 - 2.60	0.30 - 2.50	mg/d
53	dehydroepiandrosterone	0.12 - 5.20	0.04 - 2.60	mg/d
54	11-ketoandrosterone	≤ 0.12	≤ 0.07	mg/d
55	11-ketoethiocholanolone	0.04 - 0.65	0.03 - 0.50	mg/d
56	11-hydroxyandrosterone	0.40 - 2.30	0.22 - 1.60	mg/d
57	11-hydroxyethiocholanolone	0.03 - 0.65	0.02 - 0.65	mg/d

		Male	Female	Unit
Specimen
Method

		Male	Female	Unit
Specimen
Method

		Male	Female	Unit
Specimen
Method

		Male	Female	Unit
Specimen
Method

		Male	Female	Unit
Specimen
Method

Critical Value

High Value	Low Value

Kyoto University Hospital

Kyoto University