Department of Clinical Laboratory, Kyoto University Hospital

[6181 / 3F040]

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Ver.18

3.Biochemical Test >> 3F.Lipids and Relates>>3F040 fatty acid, 4-fractionation

fatty acid, 4-fractionation

0

Standard Code (JLAC10)

1	Analyte	3F040	non-esterified fatty acids, 4-fractionation
	Identification	0000
	Specimen	022	plasma
	Method	210	other chromatographies
Result Identifying 01		3F095	62	dihomo-gamma-linolenic acid
Result Identifying 02		3F095	61	arachidonic acid
Result Identifying 03		3F095	60	eicosapentaenoic acid
Result Identifying 04		3F095	66	docosahexaenoic acid
Result Identifying 05		3F095	73	EPA/AA ratio
Result Identifying 06		00
Result Identifying 07		00

Specimen

Top	Colour	Collected Sample	Quantity	Measured Material	Required
DarkGreen Top		blood	4 mL	plasma (with heparin sodium)	0.5 mL

Specimen Rejection Criteria

haemolysis
clotted
lipaemia
inappropriate sample volume
wrong container
urine samples without the appropriate preservative
received ambient
unprotected from light
opening tubes
incterus

Specimen Stability Information

Specimen Type	Container	Temperature	Stability
plasma with sodium heparin		Frozen

Turnaround Time (TAT)

4 - 5 days

Laboratory

outsourcing (LSI Mediennce Corporation)weekday (8:30 a.m. - 5:00 p.m.)
night time or weekend

Reference Value

Period / Age

		Male	Female	Unit
Specimen		blood
Method		liquid chromatography-tandem mass spectrometry (LC-MS/MS)
62	dihomo-gamma-linolenic acid	23 - 72	23 - 72	μg/mL
61	arachidonic acid	142 - 307	142 - 307	μg/mL
60	eicosapentaenoic acid	12 - 112	12 - 112	μg/mL
66	docosahexaenoic acid	51 - 185	51 - 185	μg/mL
73	EPA/AA ratio	0.06 - 0.44	0.06 - 0.44
00	DHA/AA ratio	0.25 - 0.81	0.25 - 0.81
00	(EPA+DHA)/AA ratio	0.30 - 1.11	0.30 - 1.11

		Male	Female	Unit
Specimen		blood
Method		liquid chromatography-tandem mass spectrometry (LC-MS/MS)
62	dihomo-gamma-linolenic acid	23 - 72	23 - 72	μg/mL
61	arachidonic acid	142 - 307	142 - 307	μg/mL
60	eicosapentaenoic acid	12 - 112	12 - 112	μg/mL
66	docosahexaenoic acid	51 - 185	51 - 185	μg/mL
73	EPA/AA ratio	0.06 - 0.44	0.06 - 0.44

		Male	Female	Unit
Specimen		blood
Method		gas chromatography / mass selective detector (GC-MSD)
62	dihomo-gamma-linolenic acid	22.6 - 72.5	22.6 - 72.5	μg/mL
61	arachidonic acid	135.7 - 335.3	135.7 - 335.3	μg/mL
60	eicosapentaenoic acid	10.2 - 142.3	10.2 - 142.3	μg/mL
66	docosahexaenoic acid	54.8 - 240.3	54.8 - 240.3	μg/mL
73	EPA/AA ratio	0.05 - 0.61	0.05 - 0.61

		Male	Female	Unit
Specimen		blood
Method
62	dihomo-gamma-linolenic acid	10.9 - 43.5	10.9 - 43.5	μg/mL
61	arachidonic acid	85.1 - 207.8	85.1 - 207.8	μg/mL
60	eicosapentaenoic acid	11.6 - 107.2	11.6 - 107.2	μg/mL
66	docosahexaenoic acid	48.6 - 152.4	48.6 - 152.4	μg/mL
73	EPA/AA ratio	0.09 - 0.75	0.09 - 0.75

		Male	Female	Unit
Specimen
Method

		Male	Female	Unit
Specimen
Method

		Male	Female	Unit
Specimen
Method

		Male	Female	Unit
Specimen
Method

Critical Value

High Value	Low Value

Kyoto University Hospital

Kyoto University